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To determine the prevalence of infections with species Ureaplasma species (SPP) and Mycoplasma hominis and the antibiotic sensitivity in women of reproductive age with inflammatory diseases.Methods:2360 samples were investigated, obtained by urethral and cervical canal scrapings of reproductive age women with pelvic inflammatory disease in the clinical diagnostic laboratory of the Regional diagnostic center of Almaty. The cultivation, identification and susceptibility testing of urogenital mycoplasmas and ureaplasmas to 9 antibiotics were conducted with the use of commercial kits.Results:Among 2360 tested samples total infection (isolated USPP, Mycoplasma hominis and mixed infection) was high (47.6%). The infection rate of Ureaplasma SPP was more than 30 times higher than the prevalence of Mycoplasma hominis (23.0% and 0.7%, respectively). Mixed infection was found in 23.8% of cases. Total infection of Ureaplasma SPP and Mycoplasma hominis was the highest in women aged 30-39 years (60.0%), less in women in the age group of 40-45 years (31.4%). Most active against Ureaplasma SPP and Mycoplasma hominis were tetracyclines. Indeed 94.1% of the isolated strains of Ureaplasma SPP and 98.2% of Mycoplasma hominis were sensitive to tetracycline. The doxycycline susceptibility of Ureaplasma SPP and Mycoplasma hominis was the highest (99.5% and 98.3%, respectively).Conclusions:A high level of pefloxacin sensitivity allows to recommend this antibiotic to prescribe for women with pelvic inflammatory disease. In light of wide spread of strains of Ureaplasma SPP and Mycoplasma hominis, resistant to ofloxacin and macrolides, we do not suggest these antibiotics to treat the infections.