Baza Publikacji Pracowników Politechniki Lubelskiej

Status:
Autorzy:	Wardak Cecylia, Paczosa-Bator Beata, Malinowski Szymon
Dyscypliny:
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Rok wydania:	2020
Wersja dokumentu:	Drukowana \| Elektroniczna
Język:	angielski
Wolumen/Tom:	116
Numer artykułu:	111199
Strony:	1 - 10
Impact Factor:	7,328
Web of Science® Times Cited:	25
Scopus® Cytowania:	33
Bazy:	Web of Science \| Scopus
Efekt badań statutowych	NIE
Materiał konferencyjny:	NIE
Publikacja OA:	NIE
Abstrakty:	angielski
	Laccase-based biosensors were successfully prepared using innovative, cheap, one-step Soft Plasma Polymerization technique by deposition of a bio-recognition layer on glassy carbon electrode and MWCNT (Multi-walled Carbon Nanotubes)-modified glassy carbon electrode. The Soft Plasma Polymerization technique is based on corona discharge of cold atmospheric plasma with close to room temperature. The presented work includes study of biosensor working conditions, optimization of the voltage value applied for corona discharge generation as well as applicability and interference studies for dopamine determination. The biosensor constructed under optimal conditions (corona discharge generated at a voltage of 3 kV and in 30 s time deposition, helium flow rate 10 L/min, laccase solution flow rate 200 mu L/min) has two linear ranges from 0.1 mu mol/dm(3) to 10 mu mol/dm(3) and from 10 mu mol/dm(3) to 50 mu mol/dm(3) with dopamine detection sensitivities of 3.63 mu Adm(3)/ mu mol and 1.33 mu Adm(3)/mu mol. Application of the MWCNT interlayer allows the dopamine detection sensitivity to be significantly increased to 22.35 mu A* dm(3)/mu mol for a linear range from 0.1 mu mol/dm(3) to 6 mu mol/dm(3). Additionally, the studied biosensors have stable and anti-interference ability. Both biosensors were successfully applied for dopamine determination in pharmaceutical preparation.

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Application of cold plasma corona discharge in preparation of laccase-based biosensors for dopamine determination

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