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The building envelopes with higher thermal resistance contribute to a better thermal protection of buildings. On the other hand, external surface temperature of walls have dropped due to a reduced heat flux through the walls which may result in increased risks of moisture condensation and creation of favorable conditions for biofilms growth. This paper therefore investigates the influence of various types of thermal insulations on hygrothermal conditions of the wall surface. Using computational modelling of coupled hea.t and moisture transport, the predicted hygrothermal patterns are confronted with the optimal zones given by isopleth growth models. Based on that it is revealed, that under climatic conditions of Brno and Hradec Kralove (Czech Republic), up to 184 favorable hours a reference year may be counted. The mineral wool under the lime plaster provides the most favorable conditions for the biofilms growth among all the investigated variations. However, the influence of the insulation type is not as significant as the weather conditions. From this point of view, Brno provides a better environment for the biofilms growth
