A tool supporting primer generation in the study of DNA methylation
Fragment książki (Rozdział w monografii)
MNiSW
20
Poziom I
| Status: | |
| Autorzy: | Żarko Agnieszka, Cielma Paweł, Plechawska-Wójcik Małgorzata |
| Dyscypliny: | |
| Aby zobaczyć szczegóły należy się zalogować. | |
| Wersja dokumentu: | Drukowana | Elektroniczna |
| Język: | angielski |
| Strony: | 71 - 83 |
| Efekt badań statutowych | NIE |
| Materiał konferencyjny: | NIE |
| Publikacja OA: | TAK |
| Licencja: | |
| Sposób udostępnienia: | Witryna wydawcy |
| Wersja tekstu: | Ostateczna wersja opublikowana |
| Czas opublikowania: | W momencie opublikowania |
| Data opublikowania w OA: | 28 grudnia 2020 |
| Abstrakty: | angielski |
| Epigenetics is one of the most dynamically growing areas of genetics. Geneticists, laboratory assistants and scientists dealing with the field of epigenetics need technological support to facilitate their work. There is a need of tools supporting the entire process of gene analysis. The paper presents the process of the generation of primers to research on DNA methylation. To support this process, a dedicated application was developed. The application is supported by researchers dealing with the methylation process, including isolated DNA strands. The analysis procedure covers processes such as generating PCR/MSP primers, comparing genes, writing antisense strands or detecting CpG islands. The research covers the case study on the generation of primers in three different sequences of isolated DNA. For the purpose of this study, three species were chosen: the human (Homo sapiens), the red fox (Vulpes vulpes), and the domestic dog (Canis lupus familiaris). The analysis was performed on MC1R gene sequences downloaded from biomedical databases (NCBI) in the form of FASTA files. The paper presents the complete process of gene sequence analysis using the developed application. The application is a unique tool allowing analysis of a broader spectrum of problems and processes operating in genomes, instead of focusing on one single subject. |
