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One of the possibilities to achieve energy neutrality of wastewater treatment plants (WWTPs) is the implementation of the anaerobic co-digestion strategy. However, a key factor in its successful implementation on the technical scale is the application of components with complementary composition to sewage sludge (SS). In the 7resent study, the influence of adding various co-substrates on the energy balance of anaerobic digestion was evaluated. The following organic wastes were used as additional components to SS: organic fraction of municipal solid waste (OFMSW) and distillery spent wash (DW) applied in two- and three-component systems. The experiments were performed in semi-flow anaerobic reactors with the volume of 40 L under mesophilic conditions (35 °C) at hydraulic retention time (HRT) of 20, 18, and 16 d. The application of substrates to SS resulted in enhancements of methane yields as compared to SS mono-digestion. The statistically significant differences were observed in tertiary mixtures at both HRT of 18 and 16 d. Therein, average values were 0.20 and 0.23 m3 kg−1VSadd at HRT of 18 and 16 d, respectively. Among all co-digestion series, the most beneficial effect on energy balance was found in 20% v/v DW presence in both two- and three-component systems at HRT of 16 d.
