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This article proposes a new method for detecting slight refractive index changes under
conditions of unknown polarization state. It is argued that an insignificant modification of the
tilted fiber Bragg grating (TFBG) structure and selecting the appropriate spectral region allows us to
accurately track changes in the refractive index. It has also been proven that the method can be easily
made insensitive to temperature and that the sensitivity to changes in the polarization plane of the
input light can be significantly reduced, which is crucial in later practical applications. Analytes in
the form of an aqueous glucose solution were used to calibrate the sensor. The proposed method,
based on perpendicular tilted fiber Bragg grating (P-TFBG), has a wide range of universality because
its development and slight modification will enable the detection of glucose, pathogens, and viruses
