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The paper proposes an innovative method of using microheaters for research on cells
in vitro. A method of local heating of a single culture well, compatible with an Electric Cell-substrate
Impedance (ECIS) system is presented. A microheater and culture well system for cell culture was
modelled. Electrical and thermal simulation of the system under operating conditions was carried
out. Correct distribution of heat was observed at the site of the cell culture suspension in the medium,
while not affecting the conditions in adjacent wells. As part of the experiment, a heating element
of nichrome (NiCr) was created using the magnetron sputtering process. Electrical and thermal
measurements of the manufactured device were carried out. It has been shown that it is possible
to establish the desired temperature over the long term. In addition, the structures made were
characterised by work stability, precision in maintaining the right temperature, and the possibility of
being controlled with high accuracy. There is a problem with the precise and reproducible carrying
out of a cell culture experiment that differs only in the process temperature. In this work, a technique
for increasing the temperature locally, in a single culture well, in a medium containing eight such wells
was proposed and analyzed. The use of this method will allow avoidance of the impact of potential
changes in parameters other than temperature on the culture. That may occur when comparing cells
grown at different temperatures by means of the ECIS (Electric Cell-substrate Impedance) method.
