Impedance Measurement for the Monitoring of In Vitro Cells Cultured in the Presence of Electromagnetic Waves
Artykuł w czasopiśmie
MNiSW
100
Lista 2023
| Status: | |
| Autorzy: | Kociubiński Andrzej, Wilczyńska Aleksandra, Mazurek Paweł, Pigoń-Zając Dominika, Małecka-Massalska Teresa, Prendecka-Wróbel Monika |
| Dyscypliny: | |
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| Rok wydania: | 2023 |
| Wersja dokumentu: | Drukowana | Elektroniczna |
| Język: | angielski |
| Numer czasopisma: | 3 |
| Wolumen/Tom: | 13 |
| Numer artykułu: | 1267 |
| Strony: | 1 - 14 |
| Web of Science® Times Cited: | 1 |
| Scopus® Cytowania: | 1 |
| Bazy: | Web of Science | Scopus |
| Efekt badań statutowych | NIE |
| Finansowanie: | This research was funded by the Lublin University of Technology under Grant FD-20/EE-2/311. |
| Materiał konferencyjny: | NIE |
| Publikacja OA: | TAK |
| Licencja: | |
| Sposób udostępnienia: | Witryna wydawcy |
| Wersja tekstu: | Ostateczna wersja opublikowana |
| Czas opublikowania: | W momencie opublikowania |
| Data opublikowania w OA: | 17 stycznia 2022 |
| Abstrakty: | angielski |
| This paper explores the possibility of using the impedance measurement method used to monitor morphological changes in culture cells for use in cultures in the presence of an electromagnetic field generated by a mobile phone. For this purpose, we used Electric Cell–Substrate Impedance Sensing (ECIS), which is a real-time, label-free, impedance-based method to study cell behaviors in tissue culture. As part of the work, a device enabling the connection in a climatic chamber was prepared without the need to interfere with environmental conditions, and a test culture of mouse fibroblasts was performed. The device based on the Arduino UNO programmable platform worked like a mobile phone. During cell proliferation, it was connected to the device three times and a change in electrical parameters in the measuring system was observed. During the phone call, there was a clear change in the values of the measured parameters. However, analysis of the obtained results indicated that there was little or no effect of the presence of the electromagnetic field on the cell culture, while the observed changes in the values of impedance, resistance, and capacitance are most likely due to the separation of positive and negative medium ions in the electromagnetic field. The application of the presented method seems possible; however, in order to eliminate the separation of ions, a different type of antenna should be designed to emit a homogeneous field to the entire well. |
