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This publication was founded in the framework of the activities of the Polish Metrological
Union are financed from the funds of the Ministry of Education and Science as part of a targeted
subsidy for the implementation of the task titled ”Establishment and Coordination of the activities
of the Polish Metrological Union (PMU)” under contract no. MEiN/2021/DPI/179. Research was
supported by; Slovak Research and Development Agency Grants: APVV-17-0382 and APVV-20-0566,
Project No. 2016/22/Z/ST8/00694; Slovak Grant Agency VEGA grants 1/0822/21 and 1/0596/22.
In the technological processes requiring mild treatment, such as soft materials processing or
medical applications, an important role is played by non-equilibrium plasma reactors with dielectric
barrier discharge (DBD), that when generated in noble gases allows for the effective treatment
of biological material at a low temperature. The aim of this study is to determine the operating
parameters of an atmospheric pressure, radio-frequency DBD plasma jet reactor for the precise
treatment of biological materials. The tested parameters were the shape of the discharge (its length
and volume), current and voltage signals, as well as the power consumed by the reactor for various
composition and flow rates of the working gas. To determine the applicability in medicine, the
temperature, pH, concentrations of H2O2, NO2− and NO3− and Escherichia coli log reduction in
the plasma treated liquids were determined. The obtained results show that for certain operating
parameters, a narrow shape of plasma stream can generate significant amounts of H2O2, allowing for
the mild decontamination of bacteria at a relatively low power of the system, safe for the treatment of
biological materials