Informacja o cookies

Zgadzam się Nasza strona zapisuje niewielkie pliki tekstowe, nazywane ciasteczkami (ang. cookies) na Twoim urządzeniu w celu lepszego dostosowania treści oraz dla celów statystycznych. Możesz wyłączyć możliwość ich zapisu, zmieniając ustawienia Twojej przeglądarki. Korzystanie z naszej strony bez zmiany ustawień oznacza zgodę na przechowywanie cookies w Twoim urządzeniu.

Publikacje Pracowników Politechniki Lubelskiej

MNiSW
140
Lista 2024
Status:
Autorzy: Trzaskowska Marta, Vivcharenko Vladyslav, Benko Aleksandra, Franus Wojciech, Goryczka Tomasz, Barylski Adrian, Pałka Krzysztof, Przekora Agata
Dyscypliny:
Aby zobaczyć szczegóły należy się zalogować.
Rok wydania: 2024
Wersja dokumentu: Drukowana | Elektroniczna
Język: angielski
Wolumen/Tom: 14
Numer artykułu: 28137
Strony: 1 - 19
Impact Factor: 3,8
Web of Science® Times Cited: 0
Scopus® Cytowania: 0
Bazy: Web of Science | Scopus
Efekt badań statutowych NIE
Finansowanie: The article was written within PRELUDIUM 20, grant No. UMO-2021/41/N/NZ7/01633 financed by the National Science Centre (NCN) in Poland.
Materiał konferencyjny: NIE
Publikacja OA: TAK
Licencja:
Sposób udostępnienia: Otwarte czasopismo
Wersja tekstu: Ostateczna wersja opublikowana
Czas opublikowania: W momencie opublikowania
Data opublikowania w OA: 15 listopada 2024
Abstrakty: angielski
Hydroxyapatite (HA) granules are frequently used in orthopedics and maxillofacial surgeries to fill bone defects and stimulate the regeneration process. Optimal HA granules should have high biocompatibility, high microporosity and/or mesoporosity, and high specific surface area (SSA), which are essential for their bioabsorbability, high bioactivity (ability to form apatite layer on their surfaces) and good osseointegration with the host tissue. Commercially available HA granules that are sintered at high temperatures (≥ 900 °C) are biocompatible but show low porosity and SSA (2–5 m2/g), reduced bioactivity, poor solubility and thereby, low bioabsorbability. HA granules of high microporosity and SSA can be produced by applying low sintering temperatures (below 900 °C). Nevertheless, although HA sintered at low temperatures shows significantly higher SSA (10–60 m2/g) and improved bioabsorbability, it also exhibits high ion reactivity and cytotoxicity under in vitro conditions. The latter is due to the presence of reaction by-products. Thus, the aim of this study was to fabricate novel biomaterials in the form of granules, composed of hydroxyapatite nanopowder sintered at a high temperature (1100 °C) and a biopolymer matrix: chitosan/agarose or chitosan/β-1,3-glucan (curdlan). It was hypothesized that appropriately selected ingredients would ensure high biocompatibility and microstructural properties comparable to HA sintered at low temperatures. Synthesized granules were subjected to the evaluation of their biological, microstructural, physicochemical, and mechanical properties. The obtained results showed that the developed nanocomposite granules were characterized by a lack of cytotoxicity towards both mouse preosteoblasts and normal human fetal osteoblasts, and supported cell adhesion to their surface. Moreover, produced biomaterials had the ability to induce precipitation of apatite crystals after immersion in simulated body fluid, which, combined with high biocompatibility, should ensure good osseointegration after implantation. Additionally, nanocomposite granules possessed microstructural parameters similar to HA sintered at a low temperature (porosity approx. 50%, SSA approx. 30 m²/g), Young’s modulus (5–8 GPa) comparable to cancellous bone, and high fluid absorption capacity. Moreover, the nanocomposites were prone to biodegradation under the influence of enzymatic solution and in an acidic environment. Additionally, it was noted that the hydroxyapatite nanoparticles remaining after the physicochemical dissolution of the biomaterial were easily phagocytosed by mouse macrophages, mouse preosteoblasts, and normal human fetal osteoblasts (in vitro studies). The obtained materials show great potential as bone tissue implantation biomaterials with improved bioresorbability. The obtained materials show great potential as bone tissue implantation biomaterials with improved bioresorbability.