Exploring mechanisms underlying the beneficial effect of whey protein isolate on the biocompatibility of the thermally obtained curdlan-based hydrogel
Artykuł w czasopiśmie
MNiSW
140
Lista 2024
| Status: | |
| Autorzy: | Klimek Katarzyna, Kierys Agnieszka, Matwijczuk Arkadiusz, Michalak Agnieszka, Ślusarczyk Lidia, Benko Aleksandra, Pałka Krzysztof, Truszkiewicz Wiesław, Tarczyńska Marta |
| Dyscypliny: | |
| Aby zobaczyć szczegóły należy się zalogować. | |
| Rok wydania: | 2025 |
| Wersja dokumentu: | Drukowana | Elektroniczna |
| Język: | angielski |
| Wolumen/Tom: | 366 |
| Numer artykułu: | 123821 |
| Strony: | 1 - 16 |
| Impact Factor: | 12,5 |
| Web of Science® Times Cited: | 1 |
| Scopus® Cytowania: | 1 |
| Bazy: | Web of Science | Scopus |
| Efekt badań statutowych | NIE |
| Finansowanie: | The research was mainly funded by the National Science Centre (NCN) in Poland in the framework of the MINIATURA 4 grant no. 2020/ 04/X/ST5/00112 entitled “Influence of protein isolate on biocompatibility improvement of curdlan-based biomaterial”. This study was also partially supported by the Ministry of Science and Higher Education in Poland within the DS3 and DS341 projects of the Medical University of Lublin, Poland and also FD/20/IM-5/078 project of Lublin of Technology, Poland. The experiments were conducted using the equipment purchased in the framework of the agreement no. POPW.01.03.00-06-010/09-00 Operational Program Development of Eastern Poland 2007–2013, Priority Axis I, Modern Economy, Operations 1.3. Innovations Promotion. The graphical abstract was created using BioR ender.com. |
| Materiał konferencyjny: | NIE |
| Publikacja OA: | TAK |
| Licencja: | |
| Sposób udostępnienia: | Witryna wydawcy |
| Wersja tekstu: | Ostateczna wersja opublikowana |
| Czas opublikowania: | W momencie opublikowania |
| Data opublikowania w OA: | 31 maja 2025 |
| Abstrakty: | angielski |
| This work aimed to compare the biomedical potential of thermally obtained hydrogels consisting of curdlan and a curdlan–whey protein isolate mixture. The purpose of this paper was also to investigate and explain the mechanisms responsible for their different cyto- and biocompatibility. To do this, mechanical and structural properties, wettability, and protein adsorption of biomaterials were analysed and compared. Biological experiments in vitro were carried out on normal human chondrocytes, osteoblasts, and macrophages. Moreover, in vivo studies were performed using the Danio rerio (zebrafish). Our studies showed that in comparison to the curdlan/whey protein isolate hydrogel, the curdlan hydrogel had lower mechanical properties. Its surface was less rough and more hydrophobic and adsorbed a greater amount of blood proteins, but most probably induced their conformational changes. In addition, the curdlan hydrogel exhibited lower cytocompatibility towards normal human osteoblasts and chondrocytes. It also induced greater inflammation in human macrophages in vitro and did not enhance the re-growth of the amputated caudal fin in the zebrafish larvae model when compared to the curdlan/whey protein isolate hydrogel. Hence, our studies demonstrated that a commonly used curdlan hydrogel has a significantly lower biomedical potential compared to the curdlan/whey protein isolate hydrogel proposed by us. |
