Management of milking wastewater as a significant control point of microbial pollution in the environment
Artykuł w czasopiśmie
MNiSW
100
Lista 2024
| Status: | |
| Autorzy: | Kasela Martyna, Ossowski Mateusz, Wlazło Łukasz, Andrzejczuk Sylwia, Widomski Marcin K., Michalska-Warias Aneta, Musz-Pomorska Anna, Suchorab Paweł, Karpińska Katarzyna, Malm Anna, Nowakowicz-Dębek Bożena |
| Dyscypliny: | |
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| Rok wydania: | 2026 |
| Wersja dokumentu: | Drukowana | Elektroniczna |
| Język: | angielski |
| Numer czasopisma: | 2 |
| Wolumen/Tom: | 33 |
| Strony: | 167 - 177 |
| Impact Factor: | 1,5 |
| Web of Science® Times Cited: | 0 |
| Scopus® Cytowania: | 0 |
| Bazy: | Web of Science | Scopus |
| Efekt badań statutowych | NIE |
| Finansowanie: | The project was financed by the Union of Lublin Universities in the framework of the program "INTERPROJECT" (Grant no.: INT/006/2023/II-N). |
| Materiał konferencyjny: | NIE |
| Publikacja OA: | TAK |
| Licencja: | |
| Sposób udostępnienia: | Otwarte czasopismo |
| Wersja tekstu: | Ostateczna wersja opublikowana |
| Czas opublikowania: | W momencie opublikowania |
| Data opublikowania w OA: | 25 sierpnia 2025 |
| Abstrakty: | angielski |
| Introduction and objective: Milking wastewater (MW) is an under-recognised reservoir of antibiotic resistant bacteria (ARB) and antimicrobial resistance genes (ARGs). The aim of the study is to evaluate MW as a point source pollutant for the presence of ARB and ARGs, to develop a treatment plant, and to address current legislation. Material and methods: Milking wastewater was analysed for the total number of bacteria, Enterococcus spp., Staphylococcus spp., Enterobacterales, and Clostridium perfringens. Microbial identification by MALDI-TOF MS was followed by antimicrobial susceptibility testing and detection of ARGs. Wastewater samples were also tested for the antimicrobials presence using immunochromatographic tests. An on-site two-chamber MW treatment plant was designed with consideration of its cost-effectiveness and profitability. The system incorporated filtration through a mixture of fine sand and 10% kaolin, or a fine sand filter bed, yielding hydraulic conductivity coefficients of 5.13 × 10–6 m/s and 5.36 × 10–5 m/s, respectively. The treated effluent was considered suitable for activities not requiring tap water quality. Results: Escherichia coli and Enterococcus spp. were identified as ARB. PCR confirmed the presence of ARGs conferring resistance to β-lactams (cphA, blaGES1–9,11, blaTEM1,2) and chloramphenicol (cfr). Milkling wastewater samples also tested positive for chloramphenicol. Conclusions: To mitigate environmental contamination, regulations should be established for the release of bioactive substances, with penalties imposed for non-compliance with recommended safety measures. |
